課程名稱 |
生物能量與代謝工程 Bioenergetics and Metabolic Engineering |
開課學期 |
107-2 |
授課對象 |
電機資訊學院 電機工程學研究所 |
授課教師 |
魏安祺 |
課號 |
BEBI5012 |
課程識別碼 |
945 U3020 |
班次 |
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學分 |
3.0 |
全/半年 |
半年 |
必/選修 |
選修 |
上課時間 |
星期五6,7,8(13:20~16:20) |
上課地點 |
明達203 |
備註 |
總人數上限:24人 |
Ceiba 課程網頁 |
http://ceiba.ntu.edu.tw/1072BEBI5012 |
課程簡介影片 |
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核心能力關聯 |
核心能力與課程規劃關聯圖 |
課程大綱
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課程概述 |
.Metabolic engineering focus on using a systems-level view to analyze cellular metabolism and predict optimal rewiring of metabolic networks. It integrates large-scale data collection, high-throughput biology and in silico modeling along with genome-wide engineering to improve cellular phenotype or pathway flux.
The first half of the class will review and consolidate concepts in bioenergetics, focusing on bioenergetics principles, mitochondria physiology and pathology, and metabolism pathways. The second half of the class will introduce metabolic network analysis, metabolic engineering and its applications. Topics will include integration of the techniques of molecular biology with the tools of mathematical analysis, to help elucidate metabolic flux control and rational selection of targets for genetic modification. |
課程目標 |
The class will introduce basic principles of bioenergetics, reviewing cellular metabolic pathways, and connecting the area of systems biology with metabolic engineering. The objective of the class is providing a quantitative basis for the understanding of metabolic networks from single cell to organ level. Students will also be expected to read recent papers in the related field, investigate one topic and make a short class presentation. |
課程要求 |
待補 |
預期每週課後學習時數 |
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Office Hours |
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指定閱讀 |
1.Bioenergetics 4 by David Nicholls (2013)
2.Systems Biology: Constraint-based Reconstruction and Analysis, 2nd ed. by Bernhard Palsson (2016)
(教師不提供教科書,請自行購買或查詢圖書館資源) |
參考書目 |
Systems Biology: Properties of Reconstructed Networks by Bernhard Palsson (2006) |
評量方式 (僅供參考) |
No. |
項目 |
百分比 |
說明 |
1. |
期末專題 |
35% |
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2. |
課堂參與 |
30% |
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3. |
期中報告 |
35% |
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週次 |
日期 |
單元主題 |
第1週 |
2/22 |
Class overview
Python/MATLAB & COBRA |
第2週 |
3/01 |
調整放假 |
第3週 |
3/08 |
Ch.1 & 2
Introduction of bioenergetics
Ion transport across energy-conserved membrnae |
第4週 |
3/15 |
Ch. 3 Quantitative bioenergetics
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第5週 |
3/22 |
Ch. 4 Chemiosmotic proton circuit
Ch. 5 Respiratory Chain
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第6週 |
3/29 |
student topics: complex I, II, III, IV, ATP synthase (Ch 5 & 7 Respiratory Chain)
Ch9 Cellular Bioenergetics |
第7週 |
4/05 |
春假 |
第8週 |
4/12 |
Ch. 10 Cell biology of mitochondria
Student topics: mitochondrial dynamics (fission and fusion), trafficking, biogenesis, mitophagy, ER/SR interaction
Ch11 Signaling pathway
(Midterm exam) |
第9週 |
4/19 |
Invited talks @1:30pm
Dr. Chih-Chiang Chan (NTU Physiology)
Dr. Chin-Hsien Lee (NTUH) |
第10週 |
4/26 |
Student oral presentation: mitochondrial pathology (Ch.12)
Cancer, brain, heart, beta cell and diabetes, stem cell, ageing, immune reponse |
第11週 |
5/03 |
Biochemical reaction networks and metabolic network reconstruction
Part I introduction |
第12週 |
5/10 |
Cellular metabolism
Metabolism student topics: carbonhydrates, lipids, amino acid,nucleotides etc. |
第13週 |
5/17 |
Part II Mathematical properties of reconstructed networks |
第14週 |
5/24 |
Part III
Determining the Phenotypic Potential of Reconstructed Networks |
第15週 |
5/31 |
Part IV
Omic data integration and analysis
Applications of metabolic engineering
(https://www.ibiology.org/bioengineering/metabolic-engineering/)
(https://www.ibiology.org/bioengineering/synthetic-biology/)
Biology for Chemical Production Symposium (中研院)
https://db1x.sinica.edu.tw/ASL/registerA.php?no=101&ln=0&sh=N |
第16週 |
6/07 |
端午節 |
第17週 |
6/14 |
Project tutorial |
第18週 |
6/21 |
Term project oral presentation |
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